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pmal c2x expression vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pmal c2x expression vector
    Pmal C2x Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmal+c2x+expression+vector/pMAL/bio_rxiv__64898__2026__03__05__709893-151-5-9
    Average 96 stars, based on 3339 article reviews
    pmal c2x expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: MaMYB4 is a negative regulator and a substrate of RING-type E3 ligases MaBRG2/3 in controlling banana fruit ripening.
    Article Snippet: Ying-Ying Yang , Wei Shan , Tian-Wei Yang , Chao-Jie Wu , Xun-Cheng Liu , Jian-Ye Chen , Wang-Jin Lu , Zheng-Guo Li , Wei Deng* and Jian-Fei Kuang* State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, China, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China, and Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 400044, China

    Article Title: Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
    Article Snippet: .. To generate BS2 antiserum, the cDNA of 667–1242 aa in S2 region was cloned into pMAL-c2x expression vector (NEB). .. BS2 protein was purified by using Amylose Resins (NEB) and then was used to immunize rabbit.

    Article Title: Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean ( Vigna radiata L.)
    Article Snippet: .. The full-length CDS sequence of each VrYUCCA gene was ligated into a pMAL-c2x expression vector (NEB, MBP-tag). .. Then, the recombinant VrYUCCA proteins were expressed in E. coli (BL21, DE3) and purified by dextrin Sepharose affinity chromatography (Amylose resin, NEB), in accordance with the instructions described previously [ ].

    Article Title: Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Immuno-epidemiology of human Schistosoma haematobium infection: preferential IgG3 antibody responsiveness to a recombinant antigen dependent on age and parasite burden
    Article Snippet: The RT-PCR product thus obtained was A-tailed with Taq polymerase, gel purified, ligated to pGEM ® -T vector (Promega) and the recombinant plasmid used to transform E. coli JM109 cells. .. Inserts were then released from plasmid DNA by Eco RI and Sal I digestion, and sub-cloned into the pMal-c2x expression vector (NEB) cut with the same enzymes. ..

    Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
    Article Snippet: .. I-23 was cloned in a pMAL C2X expression vector (New England Biolabs) and transformed into BL21(DE3) T1R E. coli . ..

    Article Title: Aedes aegypti lachesin protein binds to the domain III of envelop protein of Dengue virus-2 and inhibits viral replication.
    Article Snippet: Dengue virus (DENV) comprises of 4 serotypes (DENV-1 to 4) and is medically one of the most important arboviruses (arthropod-borne virus).. DENV infection is a major human health burden and is transmitted between humans by the insect vector, Aedes aegypti.. Ae. aegypti ingests DENV while feeding on infected humans, which traverses through its gut, haemolymph and salivary glands of the mosquito before being injected into a healthy human.

    Clone Assay:

    Article Title: Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
    Article Snippet: .. To generate BS2 antiserum, the cDNA of 667–1242 aa in S2 region was cloned into pMAL-c2x expression vector (NEB). .. BS2 protein was purified by using Amylose Resins (NEB) and then was used to immunize rabbit.

    Article Title: Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
    Article Snippet: .. I-23 was cloned in a pMAL C2X expression vector (New England Biolabs) and transformed into BL21(DE3) T1R E. coli . ..

    Article Title: Aedes aegypti lachesin protein binds to the domain III of envelop protein of Dengue virus-2 and inhibits viral replication.
    Article Snippet: Dengue virus (DENV) comprises of 4 serotypes (DENV-1 to 4) and is medically one of the most important arboviruses (arthropod-borne virus).. DENV infection is a major human health burden and is transmitted between humans by the insect vector, Aedes aegypti.. Ae. aegypti ingests DENV while feeding on infected humans, which traverses through its gut, haemolymph and salivary glands of the mosquito before being injected into a healthy human.

    Plasmid Preparation:

    Article Title: Virus-Like Particles of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
    Article Snippet: .. To generate BS2 antiserum, the cDNA of 667–1242 aa in S2 region was cloned into pMAL-c2x expression vector (NEB). .. BS2 protein was purified by using Amylose Resins (NEB) and then was used to immunize rabbit.

    Article Title: Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean ( Vigna radiata L.)
    Article Snippet: .. The full-length CDS sequence of each VrYUCCA gene was ligated into a pMAL-c2x expression vector (NEB, MBP-tag). .. Then, the recombinant VrYUCCA proteins were expressed in E. coli (BL21, DE3) and purified by dextrin Sepharose affinity chromatography (Amylose resin, NEB), in accordance with the instructions described previously [ ].

    Article Title: Immuno-epidemiology of human Schistosoma haematobium infection: preferential IgG3 antibody responsiveness to a recombinant antigen dependent on age and parasite burden
    Article Snippet: The RT-PCR product thus obtained was A-tailed with Taq polymerase, gel purified, ligated to pGEM ® -T vector (Promega) and the recombinant plasmid used to transform E. coli JM109 cells. .. Inserts were then released from plasmid DNA by Eco RI and Sal I digestion, and sub-cloned into the pMal-c2x expression vector (NEB) cut with the same enzymes. ..

    Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
    Article Snippet: .. I-23 was cloned in a pMAL C2X expression vector (New England Biolabs) and transformed into BL21(DE3) T1R E. coli . ..

    Article Title: Aedes aegypti lachesin protein binds to the domain III of envelop protein of Dengue virus-2 and inhibits viral replication.
    Article Snippet: Dengue virus (DENV) comprises of 4 serotypes (DENV-1 to 4) and is medically one of the most important arboviruses (arthropod-borne virus).. DENV infection is a major human health burden and is transmitted between humans by the insect vector, Aedes aegypti.. Ae. aegypti ingests DENV while feeding on infected humans, which traverses through its gut, haemolymph and salivary glands of the mosquito before being injected into a healthy human.

    Sequencing:

    Article Title: Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean ( Vigna radiata L.)
    Article Snippet: .. The full-length CDS sequence of each VrYUCCA gene was ligated into a pMAL-c2x expression vector (NEB, MBP-tag). .. Then, the recombinant VrYUCCA proteins were expressed in E. coli (BL21, DE3) and purified by dextrin Sepharose affinity chromatography (Amylose resin, NEB), in accordance with the instructions described previously [ ].

    other:

    Article Title: A Kink in DWORF Helical Structure Controls the Activation of the Sarco-plasmic Reticulum Ca 2+ -ATPase
    Article Snippet: The human DNA sequence for DWORF (NCBI Accession NP_001339058.1) was obtained from GeneWiz (South Plainfield, New Jersey) codon optimized for E. coli and ligated into a pMALTM c2X expression vector (Ampicillin resistant; New England BioLabs) by EcoRI and HindIII restriction sites.

    Binding Assay:

    Article Title: Expression and copper binding properties of the N-terminal domain of copper P-type ATPases of African trypanosomes.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Transformation Assay:

    Article Title: Miniprotein inhibitors of the Staphylococcus aureus efflux transporter NorA
    Article Snippet: .. I-23 was cloned in a pMAL C2X expression vector (New England Biolabs) and transformed into BL21(DE3) T1R E. coli . ..

    Synthesized:

    Article Title: Aedes aegypti lachesin protein binds to the domain III of envelop protein of Dengue virus-2 and inhibits viral replication.
    Article Snippet: Dengue virus (DENV) comprises of 4 serotypes (DENV-1 to 4) and is medically one of the most important arboviruses (arthropod-borne virus).. DENV infection is a major human health burden and is transmitted between humans by the insect vector, Aedes aegypti.. Ae. aegypti ingests DENV while feeding on infected humans, which traverses through its gut, haemolymph and salivary glands of the mosquito before being injected into a healthy human.



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